How does rice pollination
Immediately after being removed from the hot water, some spikelets should open. One-third of the glume must be clipped off of each spikelet, and remaining spikelets that have not opened are cut off with a pair of sharp scissors.
At least emasculated spikelets are needed for a successful cross. Once the emasculation is finished, a glassine cross bag is immediately placed over the panicle with the female label on the bag along with the date. Plants can be placed back into the greenhouse awaiting pollination. Emasculation is also carried out by using a small vacuum pump to extract the unripe anthers from spikelets of selected female parents by suction force. The device contains an oil-free pump, plastic tube and filters for filtering pollen grains and incoming air.
A glass pipette is connected to the end of the plastic tube, and the narrow nozzle of the pipette is used for sucking anthers from the clipped spikelets. A panicle of the designated female parent with only a few top spikelets that flowered the day before is the perfect material for vacuum emasculation.
The panicle is trimmed from the bottom upward to leave well-spaced spikelets to be emasculated. An opening is then cut on each of the selected spikelets separately so that the anthers come free to be vacuumed out of the spikelet. The tip of the pipette is inserted into the clipped spikelet, and the vacuum sucks out anthers without damaging the stigma.
The emasculated panicle is then covered with a labeled glassine cross bag and the plant is moved back into the greenhouse and is now ready for pollination. The process of transferring pollen from selected male parents to the emasculated spikelets of female parents is called pollination.
It may be done immediately after emasculation or may be delayed for up to several days. It can be done either by bagging a flowering male panicle above the emasculated female panicle in a glassine cross bag or by cutting off the entire or part of a flowering male panicle and swirling around the female inside the glassine cross bag. Nature , 8 Download citation. Published : 02 July Issue Date : 03 July Anyone you share the following link with will be able to read this content:.
Sorry, a shareable link is not currently available for this article. Provided by the Springer Nature SharedIt content-sharing initiative. Advanced search. Skip to main content Thank you for visiting nature. This facilitates opening of spikelets. After minutes, wet cloth is removed and spikelets are found to be open. Then, the six anthers are removed. This works on the principle of suction pressure. The spikelets are clipped off prior to operation. The minute pipette is to be shown at the point of clipping and pollen is sucked in.
Six panicles can be emasculated at a time. By hand emascualtion, flowers can be emasculated by a person. The panicle to be emasculated is inserted into hallow piece of bamboo closed at one end and plugged with cotton wool and split cork at the other end.
The flowers thus enclosed will open within minutes. The anters are removed. The panicles are enclosed in a Brown paper cover before a couple of hours of blooming.
Heat develops inside due to which the anthers extrude, but donot dehisce. This happens in minute then the anthers are easily clipped off. Stigmatic surface is then dusted with pollen grains collected from the chosen male parent. The crossed panicle is then properly tagged and protected with paper cover which is retained in a position for 7 — 10 days.
In this method hot water is kept in the flask and it is poured outside. After pouring out the water inside of the flask will be warm and humid. The panicle to be emasculated will be inserted into the flask and kept for some time.
Due to high temperature and humidity the spikelets will get opened and the anthers are exposed which can be removed with the help of forceps. Content on this page requires a newer version of Adobe Flash Player.
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