Why blocking buffer

Our solutions can help develop more accurate and long-lasting results that drive scientific innovation. Our products are dependable and high quality. Compromise elsewhere. Use Rockland blocking buffers to boost your results today. Western Blot or immunoblot is a work horse immunoassay for most labs, used to demonstrate the presence or absence of important proteins, detect post-translational modifications, diagnose disease, and more.

Robust bands that identify targets can be visualized after probing your Western blot with Rockland primary antibodies for detection and conjugated secondary antibodies. The proprietary formulation of the solution ensures high stripping efficiency with low backgrounds. It is faster and more efficient at stripping primary and secondary antibodies, allowing blots to be stripped multiple times for the repeated use of membranes.

This solution can be used at room temperature and only requires 5—20 minutes of incubation time to strip the membrane. Rockland phospho blocking buffers reduce the background noise of particular assay types. Rockland remains a leader in blocking buffer development, maintaining continuous quality control and process management throughout production.

In these situations, a blocking buffer containing BSA may be preferred. Or, to avoid potential cross-reactivity between antibodies and protein blocking agents, a non-protein blocking such as PVP can be used.

Besides the blocking agent, other blocking buffer components such as detergents and buffer salts can also influence interactions between the primary antibody and its target.

Western blot blocking agent. Top Navigation My Account. Quantitative Westerns: What is the best way to normalize your Western blot? Services Support Contact. Choosing the right blocker is important because: Insufficient blocking yields high background and reduced signal-to-noise ratios. Excessive blocking may cause loss of blotted proteins 1 or mask antibody:antigen interactions. No single blocking buffer selection is suitable for ALL antigen-antibody pairs.

Blocker choice may affect antibody specificity and non-specific binding. Intercept Protein-Based Blocking Buffers Intercept non-mammalian protein-based blocking buffers provide the same highly efficient blocking with high signal-to-noise ratio in immunoassays as Odyssey Blocking Buffers, but in a new, cost-effective formula.

Intercept Protein-Free Blocking Buffers Protein-free blocking buffers are essential for applications where animal-based products are prohibited or protein-based blocking buffers are contributing to cross-reactivity.